Seperation and determination of anticoagulant cumarins which have different therapeutical effects by HPLC

The separation and determination of some anticoagulants drugs like cumarins derivatives were done directly by high performance liquid chromatography reverse phase.We got two separate mobile phases in different percentages, the first one is water 5%: methanol 95%, and the second is water 20%: acetonitril 80% with PH = 4 for both mobile phases. The steady phase was a column of octadecyle 5 micrometre [250mm X 46mm] Nucleosil, then we have got the best results of separation and retention time and determination of the concentrations using different wave lengths of U. V as a detector, that there was a special maximum wave length for each cumarin derivative, where coefficient variation was between 0.04%-3.9% and the limit of detection was between 0.3-1.9 microgram/ml for studied cumarins depending on the mobile phase and cumarin derivative. The assay method was used to qualify the purity of studied cumarin compounds especially used in human uses and quantify and separate it of other compounds in addition to toxicity cases. This method is accurate and sensitive based on quantitative studies

Seperation and determination of anticoagulant cumarins HPLC

The separation and determination of some anticoagulants drugs and cumarins derivatives sodium warfarin were done directly by high performance liquid chromatography reverse phase [where steady phase is less polar than mobile phase]. We used two separate mobile phases in different percentages, the first one is water: methanol, and the second is water: acetonitril with PH = 4 till we got the best percentages. The steady phase was a column of octadecyle 5 micron Nucleosil [250 mmx 46mm], then we've got the best separation and retention time and determination of the concentrations using maximum wave length of U.V 283n.m for sodium warfarin [cumarin derivative], where variation coefficient was 0.12%-3.5% and the limit of detection was 1.9-0.5 micro g/ml. The assay method was used to qualify the purity of studied sodium warfarin used in human uses as an anticoagulant and quantify and separate it of other compounds in tablets in pharmaceutical companies. Consequently our results showed a very good correlation and they were useful for quality control in the drug industries